Atherogenic Diet Accelerates Ectopic Mineralization in a Mouse Model of Pseudoxanthoma Elasticum.

ABSTRACT: Objective:Pseudoxanthoma elasticum (PXE) is a multisystem heritable disorder caused by mutations in the Abcc6 gene. The disease is characterized by ectopic mineralization of the skin, eyes, and arterial blood vessels. Previous studies have suggested that cardiovascular complications in patients with PXE are caused in part by premature atherosclerosis. The aim of this study is to determine the effect of an atherogenic diet on ectopic mineralization. Methods:We used Abcc6 tm1JfK mice (Abcc6 -/- mice) as an established preclinical model of PXE. The offspring at age of 4 weeks were divided into two groups and fed the standard control laboratory diet (control group) and the atherogenic diet. Serum lipid profiles and bile acids were measured, and steatosis and tissue mineralization were evaluated by histopathologic analysis and chemical calcium quantification assay, respectively. Results:After 50-58 weeks of feeding an atherogenic diet, the concentrations of total cholesterol, low-density lipoprotein/very-low-density lipoprotein cholesterol, and bile acids were significantly higher in the Abcc6 -/- mice on the atherogenic diet (180.9?±?14.8?g/L, 145.9?±?12.9?g/L, and 9.7?±?1.4??mol/L, respectively) than in Abcc6 -/- mice on a control diet (85.2?±?4.8?g/L, 25.1?±?5.5?g/L, and 3.3?±?0.5??mol/L, respectively) (P?

SUBMITTER: Zhao JY 

PROVIDER: S-EPMC7446280 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature