Ontology highlight
ABSTRACT:
SUBMITTER: Raub CB
PROVIDER: S-EPMC7446286 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Raub Christopher B CB Lee Chen-Chung CC Shibata Darryl D Taylor Clive C Kartalov Emil E
Analytical chemistry 20160212 5
We report on HistoMosaic, a novel technique for genetic analysis of formalin-fixed, paraffin-embedded tissue slices. It combines microfluidic compartmentalization, in situ allele-specific PCR, and fluorescence microscopy. The experimental proof of principle was achieved by in situ detection of KRAS G12V mutation in colorectal cancer tissues and is presented herein. HistoMosaic offers the ability to detect mutations over the entire tissue slide simultaneously, rapidly, economically, and without s ...[more]