Dataset Information
MiRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis.
Ontology highlight
ABSTRACT: Background
miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis.Methods
Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN.Results
We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN was identified as a direct gene target and its exogenous expression inhibited the pro-cancer effects of miR-425-5p. Through its ability to down-regulate PTEN, miR-425-5p activated the PI3K/AKT axis.Conclusion
We conclude that miR-425-5p promotes LCa tumorigenesis through PTEN/PI3K/AKT signaling.
SUBMITTER: Zhou JS
PROVIDER: S-EPMC7447575 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis.
BMC pulmonary medicine 20200824 1
<h4>Background</h4>miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis.<h4>Methods</h4>Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase repor ...[more]