Ontology highlight
ABSTRACT:
SUBMITTER: Juszkiewicz S
PROVIDER: S-EPMC7447978 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Juszkiewicz Szymon S Speldewinde Shaun H SH Wan Li L Svejstrup Jesper Q JQ Hegde Ramanujan S RS
Molecular cell 20200623 4
Translating ribosomes that slow excessively incur collisions with trailing ribosomes. Persistent collisions are detected by ZNF598, a ubiquitin ligase that ubiquitinates sites on the ribosomal 40S subunit to initiate pathways of mRNA and protein quality control. The collided ribosome complex must be disassembled to initiate downstream quality control, but the mechanistic basis of disassembly is unclear. Here, we reconstitute the disassembly of a collided polysome in a mammalian cell-free system. ...[more]