Ontology highlight
ABSTRACT:
SUBMITTER: Mercuri PS
PROVIDER: S-EPMC7449184 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Mercuri Paola Sandra PS Esposito Roberto R Blétard Sylvie S Di Costanzo Stefano S Perilli Mariagrazia M Kerff Frédéric F Galleni Moreno M
Antimicrobial agents and chemotherapy 20200820 9
Analysis of the genome sequence of <i>Yersinia mollaretii</i> ATCC 43969 identified the <i>bla</i><sub>YEM</sub> gene, encoding YEM-1, a putative subclass B2 metallo-β-lactamase. The objectives of our work were to produce and purify YEM-1 and to complete its kinetic characterization. YEM-1 displayed the narrowest substrate range among known subclass B2 metallo-β-lactamases, since it can hydrolyze imipenem, but not other carbapenems, such as biapenem, meropenem, doripenem, and ertapenem, with hig ...[more]