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A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo.

ABSTRACT: Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system.

SUBMITTER: Li Y

PROVIDER: S-EPMC7449546 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo.

Li Yuyao Y Song Heng H Xue Chenghong C Fang Zhijun Z Xiong Liqin L Xie Hexin H

Chemical science 20200513 23

Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system. ...[more]

PMID: 32874510

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