Adult human circulating CD34?Lin?CD45?CD133? cells can differentiate into hematopoietic and endothelial cells.

ABSTRACT: A precise identification of adult human hemangioblast is still lacking. To identify circulating precursors having the developmental potential of the hemangioblast, we established a new ex vivo long-term culture model supporting the differentiation of both hematopoietic and endothelial cell lineages. We identified from peripheral blood a population lacking the expression of CD34, lineage markers, CD45 and CD133 (CD34?Lin?CD45?CD133? cells), endowed with the ability to differentiate after a 6-week culture into both hematopoietic and endothelial lineages. The bilineage potential of CD34?Lin?CD45?CD133? cells was determined at the single-cell level in vitro and was confirmed by transplantation into NOD/SCID mice. In vivo, CD34?Lin?CD45?CD133? cells showed the ability to reconstitute hematopoietic tissue and to generate functional endothelial cells that contribute to new vessel formation during tumor angiogenesis. Molecular characterization of CD34?Lin?D45?CD133? cells unveiled a stem cell profile compatible with both hematopoietic and endothelial potentials, characterized by the expression of c-Kit and CXCR4 as well as EphB4, EphB2, and ephrinB2. Further molecular and functional characterization of CD34?Lin?CD45?CD133? cells will help dissect their physiologic role in blood and blood vessel maintenance and repair in adult life.

SUBMITTER: Ciraci E 

PROVIDER: S-EPMC7450794 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature