Dataset Information

FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate.

ABSTRACT: Plant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spectroscopy and Reversed-phase high-performance liquid chromatography (RP-HPLC) combined with Multivariate analysis (MVA) were used to detect significant differences in the PC production in cultures treated with two elicitors. The results suggest that the FT-NIR-MVA is useful for discriminating samples according to the treatment, showed significant influence of the PC signal. RP-HPLC-MVA showed that the elicitor effect occurs at 72?h post-elicitation. Detection of dihydroquercetin (maximum concentration?=?12.59?mg/L), a flavonoid with anti-cancer properties, is highlighted. Future studies will be aimed at scaling this culture to increase the productivity of dihydroquercetin.

SUBMITTER: Mendoza D

PROVIDER: S-EPMC7451858 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate.

Mendoza Dary D Arias Juan Pablo JP Cuaspud Olmedo O Ruiz Orlando O Arias Mario M

Biotechnology reports (Amsterdam, Netherlands) 20200812

Plant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spect ...[more]

PMID: 32874946

Similar Datasets

Project description:BackgroundThevetia peruviana (Pers.) K. Schum or Cascabela peruviana (L.) Lippold (commonly known as ayoyote, codo de fraile, lucky nut, or yellow oleander), native to Mexico and Central America, is a medicinal plant used traditionally to cure diseases like ulcers, scabies, hemorrhoids and dissolve tumors. The purpose of this study was to evaluate the cytotoxic, antiproliferative and apoptotic activity of methanolic extract of T. peruviana fruits on human cancer cell lines.MethodsThe cytotoxic activity of T. peruviana methanolic extract was carried out on human breast, colorectal, prostate and lung cancer cell lines and non-tumorigenic control cells (fibroblast and Vero), using the MTT assay. For proliferation and motility, clonogenic and wound-healing assays were performed. Morphological alterations were monitored by trypan blue exclusion, as well as DNA fragmentation and AO/EB double staining was performed to evaluate apoptosis. The extract was separated using flash chromatography, and the resulting fractions were evaluated on colorectal cancer cells for their cytotoxic activity. The active fractions were further analyzed through mass spectrometry.ResultsThe T. peruviana methanolic extract exhibited cytotoxic activity on four human cancer cell lines: prostate, breast, colorectal and lung, with values of IC50 1.91 ± 0.76, 5.78 ± 2.12, 6.30 ± 4.45 and 12.04 ± 3.43 μg/mL, respectively. The extract caused a significant reduction of cell motility and colony formation on all evaluated cancer cell lines. In addition, morphological examination displayed cell size reduction, membrane blebbing and detachment of cells, compared to non-treated cancer cell lines. The T. peruviana extract induced apoptotic cell death, which was confirmed by DNA fragmentation and AO/EB double staining. Fractions 4 and 5 showed the most effective cytotoxic activity and their MS analysis revealed the presence of the secondary metabolites: thevetiaflavone and cardiac glycosides.ConclusionT. peruviana extract has potential as natural anti-cancer product with critical effects in the proliferation, motility, and adhesion of human breast and colorectal cancer cells, and apoptosis induction in human prostate and lung cancer cell lines, with minimal effects on non-tumorigenic cell lines.

Project description:Copper is an essential micronutrient; however, as a result of its increasing demand, subsequent mining followed by its direct discharge into the environment has led to the contamination of our ecosystem. Thevetia peruviana (TP) is an ornamental herb of medicinal interest and is extensively used as an antipyretic and anticancer agent due to the presence of cardiac glycosides. In this work, we have explored the TP leaf powder as a biosorbent for Cu(II) removal from aqueous media and observed that it yields better results in comparison to other reported biosorbents for the removal of Cu(II). This work also emphasizes on the biosorption kinetics along with its plausible mechanism of interactions. The leaf powder characterized by FT-IR spectroscopy confirmed the diverse surface functionalities including hydroxyl, carbonyl, glycosides, etc. The morphology and elemental composition of the plant material have been investigated using SEM-EDAX analysis that confirms the heterogeneity and porosity of the biosorbent surface. The encouraging results revealed that the TP leaf powder could be used as a cost-effective biosorbent with an adsorption capacity of 187.51 mg g-1 for Cu(II) in aqueous media at pH ? 5 and a temperature of 303 K. The complex functionality of the TP surface most likely played a significant role in attaining fast equilibrium within 60 min by following pseudo-second-order kinetics, having a rate constant of 2 × 103 mg g-1 min-1 that has been confirmed with statistical tools such as regression coefficient, chi-squared, and sum of error square tests. The adsorption mechanism is controlled by diffusion of Cu(II) from the liquid phase to the solid phase of the TP biosorbent followed by the chemical interaction between the biosorbent and the adsorbate with slow intraparticle diffusion on the biosorbent surface. The adsorption of Cu(II) on TP has been observed to rise from 59.29 to 197.63 mg g-1 with the rise in the pH of the medium from 2 to 7. The adsorption of Cu(II) has been found to increase from 176.80 to 191.33 mg g-1 with increasing temperature from 293-308 K, confirming the endothermic nature of the adsorption process. The thermodynamic study revealed the adsorption process to be spontaneous with negative ?G (-10.43 to -13.74 kJ mol-1) and that it has an endothermic nature with positive ?H (54.24 kJ mol-1). The isotherm study for Cu(II) on TP followed the Langmuir adsorption isotherm model with the maximum monolayer adsorption capacity of 303.03 mg g-1 rather than Freundlich and Temkin isotherm models, which confirmed the chemical interaction between the sorbent and sorbate. FT-IR and SEM-EDAX analyses have also been used to confirm the adsorption of Cu(II) onto the TP surface. The present study revealed 99.7% Cu(II) desorption using 0.8 N HCl as the desorbent accompanied by a 69.71% regeneration efficiency of the TP biosorbent. After desorption of Cu(II), the regenerated TP could be disposed of in soil. The encouraging results revealed that TP could be used as an alternative and low-cost biosorbent for the removal of heavy metals from aqueous solutions.

Dataset Information

FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate.

Publications

FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of <i>Thevetia peruviana</i> treated with salicylic acid and methyl jasmonate.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets