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Bioremediation of toxic metals in mining site of Zamfara metropolis using resident bacteria (Pantoea agglomerans): A optimization approach.

ABSTRACT: Background:Various clean-up techniques for heavy metals have been suggested and practiced for its biosorption from the contaminated or pollutant soil by using chemical and physical methods. But most of the methods are hazardous to the environment and expensive. This study was on how to determine the potential of resident bacteria in the removal of heavy metals from contaminated soils in Abare situated in Anka Local Government of Zamfara State, Nigeria. Thus, this study employed bioremediation technique for removal of heavy metals. Results:The preparation of Culture media and Isolation of bacteria of the different contaminated soils were achieved by spread plate method. Whereas, concentrations of the heavy metals (Lead (Pb), Copper (Cu) and Iron (Fe)) were determined by Atomic absorption spectrophotometer (AAS. Pantoea agglomerans was used for biosorption experiment. The concentrations of Pb ranged between 1.328 ± 0.493 to 2.326 ± 2.093 mg/L, Cu 0.234 ± 0.117 to 1.054 ± 1.486 mg/L and Fe 18.498 ± 11.462 to 27.754 ± 57.510 mg/L. The optimum temperature for biosorption condition was found to be 35 °C. More so, the optimum pH of (7) was observed for maximum biosorption of Pb and Cu ions by Pantoea agglomerans which may be attributed to homeostatic phenomenon and the availability of metal binding sites on the biosorbents. Metal uptake biosorption percentage revealed that Pantoea agglomerans absorbed 99.6% of Pb, 60% of Cu and 96% of Fe. Conclusion:This study revealed that Pantoea agglomerans potential for bioremediation of the three metals.

SUBMITTER: Audu KE

PROVIDER: S-EPMC7452395 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Bioremediation of toxic metals in mining site of Zamfara metropolis using resident bacteria (<i>Pantoea agglomerans</i>): A optimization approach.

Audu Kalen Ephraim KE Adeniji Shola Elijah SE Obidah John Solomon JS

Heliyon 20200814 8

<h4>Background</h4>Various clean-up techniques for heavy metals have been suggested and practiced for its biosorption from the contaminated or pollutant soil by using chemical and physical methods. But most of the methods are hazardous to the environment and expensive. This study was on how to determine the potential of resident bacteria in the removal of heavy metals from contaminated soils in Abare situated in Anka Local Government of Zamfara State, Nigeria. Thus, this study employed bioremedi ...[more]

PMID: 32904083

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Project description:BACKGROUND: Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. RESULTS: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. CONCLUSION: Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this species.

Dataset Information

Bioremediation of toxic metals in mining site of Zamfara metropolis using resident bacteria (Pantoea agglomerans): A optimization approach.

Publications

Bioremediation of toxic metals in mining site of Zamfara metropolis using resident bacteria (<i>Pantoea agglomerans</i>): A optimization approach.

Similar Datasets

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