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Engaging inexpensive hands-on activities using Chlamydomonas reinhardtii (a green micro-alga) beads to teach the interplay of photosynthesis and cellular respiration to K4-K16 Biology students.

ABSTRACT: Background:Photosynthesis and cellular respiration play major roles in energy metabolism and are important Life Science topics for K16 Biology students. Algae beads are used for photosynthesis and cellular respiration labs. Currently there are a few companies that sell biology educational kits for making algae beads using non-motile green micro-algae to introduce students to photosynthesis. These kits are expensive and, do not come with detailed guidelines for trouble shooting and customizations for different grade levels. Chlamydomonas reinhardtii is a motile green micro-alga and is an excellent model system for photosynthesis studies. In this article, we are presenting the work conducted in the student-driven, American Society of Plant Biologists-funded, Plant-BLOOME educational outreach project. This project is a supervised collaborative effort of three undergraduates and one high school student. We have generated a protocol which can be used to make Chlamydomonas beads. We have used these beads to design two simple and inexpensive plant biology hands-on activities. These laboratory activities have been customized to teach the interplay of photosynthesis and cellular respiration to K4-K16 Biology students. Methods:Chlamydomonas beads were used for two different laboratory activities that involved monitoring pH changes over time using a pH indicator. Our first activity centers on making and, using light-powered algae bead bracelets to monitor dramatic color/pH changes over time when exposed to darkness or light. Our second activity employs strain-specific algae beads with approximately equal cell numbers to conduct comparative photosynthesis and cellular respiration studies in two Chlamydomonas strains namely, wild type, 4A+ and, a high light-sensitive, photosynthetic mutant, 10E35/lsr1a. Results:We optimized our experimental protocol using algae beads in a 5.5 mL screw capped glass vials before performing the same experiment in algae bead bracelets. We found that the algal cell density/bead, water type used in the experiment and, the duration of dark exposure of algal beads can affect successful implementation of the lab activities. Light-powered algae bead bracelets showed dramatic color/pH changes within 3 h upon exposure to light or darkness. These bracelets could be switched back and forth between darkness and light multiple times within 48-72 h to display color/pH changes, provided prior dark exposure time did not exceed 9 h. Our comparative studies of photosynthesis and cellular respiration in 10E35 and in 4A+ showed that relative respiration rate and photosynthetic rate is higher and lower in 10E35, respectively, compared to that in 4A+. Additionally, 10E35 failed to display the expected photosynthesis-induced pH/color changes in the light after prolonged exposure to darkness which indicated that prolonged dark exposure of 10E35, hindered photosynthesis.

SUBMITTER: Mitra M

PROVIDER: S-EPMC7453928 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Engaging inexpensive hands-on activities using <i>Chlamydomonas reinhardtii</i> (a green micro-alga) beads to teach the interplay of photosynthesis and cellular respiration to K4-K16 Biology students.

Mitra Mautusi M Broom Sara Michelle SM Pinto Kysis K Wellons Sovi-Mya Doan SD Roberts Ariel Dominique AD

PeerJ 20200825

<h4>Background</h4>Photosynthesis and cellular respiration play major roles in energy metabolism and are important Life Science topics for K16 Biology students. Algae beads are used for photosynthesis and cellular respiration labs. Currently there are a few companies that sell biology educational kits for making algae beads using non-motile green micro-algae to introduce students to photosynthesis. These kits are expensive and, do not come with detailed guidelines for trouble shooting and custom ...[more]

PMID: 32904417

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Project description:BACKGROUND AND AIMS: Under stress-promoting conditions unicellular algae can undergo programmed cell death (PCD) but the mechanisms of algal cellular suicide are still poorly understood. In this work, the involvement of caspase-like proteases, DNA cleavage and the morphological occurrence of cell death in wasp venom mastoparan (MP)-treated Chlamydomonas reinhardtii were studied. METHODS: Algal cells were exposed to MP and cell death was analysed over time. Specific caspase inhibitors were employed to elucidate the possible role of caspase-like proteases. YVADase activity (presumably a vacuolar processing enzyme) was assayed by using a fluorogenic caspase-1 substrate. DNA breakdown was evaluated by DNA laddering and Comet analysis. Cellular morphology was examined by confocal laser scanning microscopy. KEY RESULTS: MP-treated C. reinhardtii cells expressed several features of necrosis (protoplast shrinkage) and vacuolar cell death (lytic vesicles, vacuolization, empty cell-walled corpse-containing remains of digested protoplast) sometimes within one single cell and in different individual cells. Nucleus compaction and DNA fragmentation were detected. YVADase activity was rapidly stimulated in response to MP but the early cell death was not inhibited by caspase inhibitors. At later time points, however, the caspase inhibitors were effective in cell-death suppression. Conditioned medium from MP-treated cells offered protection against MP-induced cell death. CONCLUSIONS: In C. reinhardtii MP triggered PCD of atypical phenotype comprising features of vacuolar and necrotic cell deaths, reminiscent of the modality of hypersensitive response. It was assumed that depending on the physiological state and sensitivity of the cells to MP, the early cell-death phase might be not mediated by caspase-like enzymes, whereas later cell death may involve caspase-like-dependent proteolysis. The findings substantiate the hypothesis that, depending on the mode of induction and sensitivity of the cells, algal PCD may take different forms and proceed through different pathways.

Dataset Information

Engaging inexpensive hands-on activities using Chlamydomonas reinhardtii (a green micro-alga) beads to teach the interplay of photosynthesis and cellular respiration to K4-K16 Biology students.

Publications

Engaging inexpensive hands-on activities using <i>Chlamydomonas reinhardtii</i> (a green micro-alga) beads to teach the interplay of photosynthesis and cellular respiration to K4-K16 Biology students.

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