Ontology highlight
ABSTRACT:
SUBMITTER: Freed NE
PROVIDER: S-EPMC7454405 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Freed Nikki E NE Vlková Markéta M Faisal Muhammad B MB Silander Olin K OK
Biology methods & protocols 20200718 1
Rapid and cost-efficient whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019, is critical for understanding viral transmission dynamics. Here we show that using a new multiplexed set of primers in conjunction with the Oxford Nanopore Rapid Barcode library kit allows for faster, simpler, and less expensive SARS-CoV-2 genome sequencing. This primer set results in amplicons that exhibit lower levels of variation in ...[more]