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A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos.

ABSTRACT: In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.

SUBMITTER: Somkhit J

PROVIDER: S-EPMC7455472 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos.

Somkhit Julie J Loyant Roxane R Brenet Alexandre A Hassan-Abdi Rahma R Yanicostas Constantin C Porceddu Mathieu M Borgne-Sanchez Annie A Soussi-Yanicostas Nadia N

Zebrafish 20200504 4

In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibit ...[more]

PMID: 32364833

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