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Molecular basis of junctional current rectification at an electrical synapse.


ABSTRACT: Rectifying electrical synapses (RESs) exist across animal species, but their rectification mechanism is largely unknown. We investigated why RESs between AVA premotor interneurons and A-type cholinergic motoneurons (A-MNs) in Caenorhabditis elegans escape circuit conduct junctional currents (I j) only in the antidromic direction. These RESs consist of UNC-7 innexin in AVA and UNC-9 innexin in A-MNs. UNC-7 has multiple isoforms differing in the length and sequence of the amino terminus. In a heterologous expression system, only one UNC-7 isoform, UNC-7b, can form heterotypic gap junctions (GJs) with UNC-9 that strongly favor I j in the UNC-9 to UNC-7 direction. Knockout of unc-7b alone almost eliminated the I j, whereas AVA-specific expression of UNC-7b substantially rescued the coupling defect of unc-7 mutant. Neutralizing charged residues in UNC-7b amino terminus abolished the rectification property of UNC-7b/UNC-9 GJs. Our results suggest that the rectification property results from electrostatic interactions between charged residues in UNC-7b amino terminus.

SUBMITTER: Shui Y 

PROVIDER: S-EPMC7455501 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Molecular basis of junctional current rectification at an electrical synapse.

Shui Yuan Y   Liu Ping P   Zhan Haiying H   Chen Bojun B   Wang Zhao-Wen ZW  

Science advances 20200703 27


Rectifying electrical synapses (RESs) exist across animal species, but their rectification mechanism is largely unknown. We investigated why RESs between AVA premotor interneurons and A-type cholinergic motoneurons (A-MNs) in <i>Caenorhabditis elegans</i> escape circuit conduct junctional currents (<i>I</i> <sub>j</sub>) only in the antidromic direction. These RESs consist of UNC-7 innexin in AVA and UNC-9 innexin in A-MNs. UNC-7 has multiple isoforms differing in the length and sequence of the  ...[more]

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