Ontology highlight
ABSTRACT:
SUBMITTER: Liu YL
PROVIDER: S-EPMC7456512 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Liu Yen-Liang YL Perillo Evan P EP Ang Phyllis P Kim Mirae M Nguyen Duc Trung DT Blocher Katherine K Chen Yu-An YA Liu Cong C Hassan Ahmed M AM Vu Huong T HT Chen Yuan-I YI Dunn Andrew K AK Yeh Hsin-Chih HC
ACS nano 20200715 7
Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT) technique that can simultaneously localize two spectrally distinct targets in three dimensions with a time resolution down to 5 ms. The dual-targets can be tracked with separation distances from 33 to 250 nm with tracking precisions of ∼15 nm (for static targets) and ∼35 nm (for freely diffusing targets). Since each target is individually localized, a wealth of data can be extracted, such as the relative 3D positio ...[more]