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Simultaneous on-chip DC dielectrophoretic cell separation and quantitative separation performance characterization.

ABSTRACT: Through integration of a MOSFET-based microfluidic Coulter counter with a dc-dielectrophoretic cell sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples including 1) binary mixtures of polystyrene beads, 2) yeast cells of continuous size distribution, and 3) mixtures of 4T1 tumor cells and murine bone marrow cells. For cells with continuous size distribution, it is found that the receiver operator characteristic analysis is an ideal method to characterize the separation performance. The characterization results indicate that dc-DEP separation performance degrades as the sorting throughput (cell sorting rate) increases, which provides insights into the design and operation of size-based microfluidic cell separation.

SUBMITTER: Sun J

PROVIDER: S-EPMC7458407 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Simultaneous on-chip DC dielectrophoretic cell separation and quantitative separation performance characterization.

Sun Jiashu J Gao Yandong Y Isaacs Richard J RJ Boelte Kimberly C KC Lin P Charles PC Boczko Erik M EM Li Deyu D

Analytical chemistry 20120201 4

Through integration of a MOSFET-based microfluidic Coulter counter with a dc-dielectrophoretic cell sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples including 1) binary mixtures of polystyrene beads, 2) yeast cells of continuous size distribution, and 3) mixtures of 4T1 tumor cells and murine bone marrow cells. For cells with continuous size distribution, it is found that the receiver operator characteristic analysis is an ideal method to charac ...[more]

PMID: 22229858

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