A liposomal RNA vaccine inducing neoantigen-specific CD4⁺ T cells augments the antitumor activity of local radiotherapy in mice.

ABSTRACT: Antigen-encoding, lipoplex-formulated RNA (RNA-LPX) enables systemic delivery to lymphoid compartments and selective expression in resident antigen-presenting cells. We report here that the rejection of CT26 tumors, mediated by local radiotherapy (LRT), is further augmented in a CD8⁺ T cell-dependent manner by an RNA-LPX vaccine that encodes CD4⁺ T cell-recognized neoantigens (CD4 neoantigen vaccine). Whereas CD8⁺ T cells induced by LRT alone were primarily directed against the immunodominant gp70 antigen, mice treated with LRT plus the CD4 neoantigen vaccine rejected gp70-negative tumors and were protected from rechallenge with these tumors, indicating a potent poly-antigenic CD8⁺ T cell response and T cell memory. In the spleens of CD4 neoantig

SUBMITTER: Salomon N 

PROVIDER: S-EPMC7458669 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature