Heterologous expression of pepper mild mottle virus coat protein encoding region and its application in immuno-diagnostics.

ABSTRACT: Pepper mild mottle virus (PMMoV), a tobamovirus of family Virgaviridae affects the quality and quantity of Capsicum. PMMoV is highly contagious, capable of transmitting through infected seeds and soil. Symptoms are more severe when crop is infected at young stage but remain unnoticed when infection takes place at maturity. Therefore, cost effective diagnostic techniques are required for timely and accurate detection of virus. In present study, coat protein encoding region of PMMoV-HP1 isolate was cloned into expression vector system, pET28a and expressed in BL21, a protease deficient strain of Escherichia coli. The PMMoV-HP1 pathotype was identified as PMMoV-P₁₂ on the basis of coat protein amino acid sequence analysis in our previous study. The overexpressi

SUBMITTER: Kumari N 

PROVIDER: S-EPMC7458984 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature