TGF-?1-Mediated FDNCR1 Regulates Porcine Preadipocyte Differentiation via the TGF-? Signaling Pathway.

ABSTRACT: Adipocyte differentiation and lipid metabolism have important regulatory effects on the quality of meat from livestock. A variety of transcription factors regulate preadipocyte differentiation. Several studies have revealed that transforming growth factor-beta (TGF-?1) may play a key role in epithelial-mesenchymal transition (EMT); however, little is known about the effects of TGF-?1 treatment on porcine preadipocytes. To explore the role of TGF-?1 in porcine adipocyte differentiation, porcine preadipocytes were treated with 10 ng/mL TGF-?1, and two libraries were constructed for RNA-seq. We chose an abundant and differentially expressed long noncoding RNA (lncRNA), which we named fat deposition-associated long noncoding RNA1 (FDNCR1), for further study. RT-qPCR was used to detect mRNA levels of genes related to adipocyte differentiation. Triglyceride assay kits were used to detect lipid droplet deposition. TGF-?1 significantly suppressed porcine preadipocyte differentiation. We identified 8158 lncRNAs in total and 39 differentially expressed lncRNAs. After transfection with FDNCR1 siRNA, the mRNA expression of aP2, C/EBP?, and PPAR? and triglyceride levels significantly increased. Transfection with FDNCR1 siRNA significantly decreased protein levels of p-Smad2/Smad2 and p-Smad3/Smad3. These results demonstrate that FDNCR1 suppresses porcine preadipocyte differentiation via the TGF-? signaling pathway.

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC7459525 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature