Dataset Information

Diet with a High Proportion of Rice Alters Profiles and Potential Function of Digesta-Associated Microbiota in the Ileum of Goats.

ABSTRACT: Effects of a high proportion of concentrate in the diet on the ileal microbiota and metabolites in small ruminants are rarely reported. This study was designed to investigate the ileal microbiota and its relationship with host metabolic function in goats and aimed to elucidate the mechanisms involving in the ileal adaptation to a diet containing a high proportion of rice. Sixteen goats were equally divided into two groups and fed a diet with a normal concentrate proportion (NC, 55% concentrate) or a high-concentrate diet (HC, 90% concentrate). Results showed that the HC diet decreased bacterial diversity and elevated the abundance of five genera (Clostridium_sensu_stricto_1, Eubacterium_nodatum_group, Ruminococcus_gauvreauii_group, Eubacterium_coprostanoligenes_group and Ruminococcus 1), but reduced the number of Anaerotruncus. Microbial functional potentials indicated that the HC diet activated the pathways related to metabolism of carbohydrate, glycan, lipid and vitamins, but inhibited the pathways associated with cell motility and signal transduction. The activities of amylase and alkaline phosphatase were greater (p < 0.05) in the intestinal digesta of the HC-fed goats. However, there were no differences in the villus height, crypt depth and the ratio of villus height to crypt depth in the ileum between the two groups. These results indicate that the HC diet alters the bacterial community and pathways related to the metabolism of dietary nutrients and cell motility and signal transduction of bacteria in the ileum of goats.

SUBMITTER: Wang K

PROVIDER: S-EPMC7460243 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Diet with a High Proportion of Rice Alters Profiles and Potential Function of Digesta-Associated Microbiota in the Ileum of Goats.

Wang Kaijun K Ren Ao A Zheng Mengli M Jiao Jinzhen J Yan Qiongxian Q Zhou Chuanshe C Tan Zhiliang Z

Animals : an open access journal from MDPI 20200724 8

Effects of a high proportion of concentrate in the diet on the ileal microbiota and metabolites in small ruminants are rarely reported. This study was designed to investigate the ileal microbiota and its relationship with host metabolic function in goats and aimed to elucidate the mechanisms involving in the ileal adaptation to a diet containing a high proportion of rice. Sixteen goats were equally divided into two groups and fed a diet with a normal concentrate proportion (NC, 55% concentrate) ...[more]

PMID: 32722283

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Project description:BackgroundNeonatal diet has a large influence on child health and might modulate changes in fecal microbiota and metabolites.ObjectivesThe aim is to investigate fecal microbiota and metabolites at different ages in infants who were breastfed (BF), received dairy-based milk formula (MF), or received soy-based formula (SF).MethodsFecal samples were collected at 3 (n = 16, 12, and 14, respectively), 6 (n = 20, 19, and 15, respectively), 9 (n = 12, 11, and 12, respectively), and 12 mo (n = 14, 14, and 15, respectively) for BF, MF, and SF infants. Infants that breastfed until 9 mo and switched to formula were considered as no longer breastfeeding at 12 mo. Microbiota data were obtained using 16S ribosomal RNA sequencing. Untargeted metabolomics was conducted using a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer. The data were analyzed using R (version 3.6.0) within the RStudio (version 1.1.463) platform.ResultsAt 3, 6, and 9 mo of age BF infants had the lowest α-diversity, SF infants had the highest diversity, and MF was intermediate. Bifidobacterium was 2.6- to 5-fold lower in SF relative to BF infants through 1 y of life. An unidentified genus from Ruminococcaceae higher in the SF (2%) than in the MF (0.4%) and BF (0.08%) infants at 3 mo of age was observed. In BF infants higher levels of butyric acid, d-sphingosine, kynurenic acid, indole-3-lactic acid, indole-3-acetic acid, and betaine were observed than in MF and SF infants. At 3 mo Ruminococcaceae was positively correlated to azelaic, gentisic, isocitric, sebacic, and syringic acids. At 6 mo Oscillospira was negatively correlated with 3-hydroxybutyric-acid, hydroxy-hydrocinnamic acid, and betaine whereas Bifidobacterium was negatively associated with 5-hydroxytryptamine. At 12 mo of age, Lachnospiraceae was negatively associated with hydroxyphenyllactic acid.ConclusionsInfant diet has a large impact on the fecal microbiome and metabolome in the first year of life.This study was registered at clinicaltrials.gov as NCT00616395.

Project description:The effects of high-condensed tannin (CT) diet combined with preslaughter stress have not been studied at the metabolome level in goats. This study was conducted to determine the effects of feeding sericea lespedeza (SL; Lespedeza cuneata), a high-CT legume, and transportation stress on plasma metabolome in goats. Uncastrated male Spanish goats (age = 8 months; BW = 26.0 ± 0.48 kg) were either fed ground 'Serala' SL hay (SER), bermudagrass (Cynodon dactylon) hay (BG), or bermudagrass hay-dewormed goats (BG-DW; Control) at 75% of intake, with a corn-based supplementation (25%) for 8 weeks (n = 12/Diet). At the end of the trial, goats were subjected to one of two stress treatments (ST): transported for 90 min to impose stress (TS) or held in pens (NTS) before slaughtering, in two replicates. Live and carcass weights, and blood samples were collected at 0, 30, 60 and 90 min of transportation or holding time (Time). The data were analyzed using MIXED procedures in SAS and metabolomics data were analyzed using the R software. When measured after ST, SER group had the lowest body weight (P < 0.05) among the three diet groups. Carcass weights were high in the BG-DW, low in SER, and intermediate in BG group. Plasma creatine concentrations decreased over Time (P < 0.01) in the TS goats in all diet groups. Meat crude protein percentages were higher (P < 0.05) in SER (22.5 ± 0.22) and BG-DW (22.3 ± 0.22) groups compared to the BG group (21.6 ± 0.22). At the metabolome level, SER group had the lowest (P < 0.05) glycine, alanine, threonine, taurine, trans-hydroxyproline, methionine, and histidine concentrations and highest (P < 0.01) lysine and citrulline concentrations among the Diet groups. Butyric acid, concentration was higher (P < 0.05) in the SER group compared to BG group. Eight medium- and long-chained acylcarnitines were higher (P < 0.05) in the BG-DW group than SER or BG groups. In general, amino acid levels decreased and acylcarnitine increased with Time (P < 0.05) in all groups. Sericea diet can be beneficial in enhancing stress coping abilities in goats due to elevated butyrate, lysine, and citrulline levels; however, SER resulted in lower energy level in goats compared to BG or BG-DW groups. Fatty acid metabolism is the main energy pathway in all groups during prolonged stress. Inclusion of certain varieties of SL in the diet must be carefully controlled to prevent possible negative effect.

Dataset Information

Diet with a High Proportion of Rice Alters Profiles and Potential Function of Digesta-Associated Microbiota in the Ileum of Goats.

Publications

Diet with a High Proportion of Rice Alters Profiles and Potential Function of Digesta-Associated Microbiota in the Ileum of Goats.

Similar Datasets

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