Ontology highlight
ABSTRACT:
SUBMITTER: Miraglia F
PROVIDER: S-EPMC7460339 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Miraglia Fabiana F Valvano Verdiana V Rota Lucia L Di Primio Cristina C Quercioli Valentina V Betti Laura L Giannaccini Gino G Cattaneo Antonino A Colla Emanuela E
Life (Basel, Switzerland) 20200811 8
Endoplasmic reticulum (ER) dysfunction is important for alpha-synuclein (αS) acquired toxicity. When targeted to the ER in SH-SY5Y cells, transient or stable expression of αS resulted in the formation of compact αS-positive structures in a small subpopulation of cells, resembling αS inclusions. Thus, because of the limitations of immunofluorescence, we developed a set of αS FRET biosensors (AFBs) able to track αS conformation in cells. In native conditions, expression in i36, a stable cell line ...[more]