¹H, ¹³C and ¹⁵N backbone resonance assignment of BRCA1 fragment 219-504.

ABSTRACT: The breast cancer susceptibility protein 1 (BRCA1) plays a central role in the suppression of human breast and ovarian cancer. Germ line mutations of the BRCA1 gene are responsible for the hereditary breast and ovarian cancer (HBOC) syndrome. Here were report ¹H, ¹³C, and ¹⁵N resonance assignments for the intrinsically disordered BRCA1 fragment 219-504, which contains important interaction sites for the proto-oncogenic transcription factor MYC as well as for p53. A nuclear magnetic resonance assignment was achieved at 18.8 T magnetic field strength using a 5D HN(CA)CONH experiment and its associated 4D H(NCA)CONH and 4D (H)N(CA)CONH experiments. ¹³C^α and ¹³C^β assignments were obtained using a 5D HabCabCONH experim

SUBMITTER: Gorka M 

PROVIDER: S-EPMC7462910 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature