The Subgingival Microbiome in Patients with Down Syndrome and Periodontitis.

ABSTRACT: To describe the subgingival microbiome of individuals with Down syndrome (DS). We conducted a cross-sectional observational study that obtained bacterial DNA samples from 50 patients with DS, 25 with periodontitis (PDS) and 25 with a healthy periodontal condition (HDS). The samples were analyzed by sequencing the 16S rRNA gene V3-V4 hypervariable region using the MiSeq System. Taxonomic affiliations were assigned using the naïve Bayesian classifier integrated in QIIME2 plugins. We evaluated the difference in bacteria abundance between the sample groups using Wilcoxon and Kruskal-Wallis tests. We evaluated the alpha diversity of the identified species using the Observed, Chao1metric, ACE and Shannon indices and evaluated beta diversity with principal coordinate analysis (registration code:

SUBMITTER: Novoa L 

PROVIDER: S-EPMC7463899 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature