Dataset Information

Comparative Study of the Effects of Salinity on Growth, Gas Exchange, N Accumulation and Stable Isotope Signatures of Forage Oat (Avena sativa L.) Genotypes.

ABSTRACT: Identifying suitable salt stress-tolerant phenotypes based on their agronomic and physiological traits remains a herculean task in forage-type oat (Avena sativa L.) breeding. This study examined the responses of six forage-type oat cultivars under four levels of saline stress over the vegetative growth cycle. Crop growth, water status-related traits and nitrogen status-related traits were analyzed in different plant parts to evaluate effective approaches for identifying salt tolerance. Plant biomass, height, tiller number and culm thickness changed substantially during salinity, but they were not precise enough for use in estimating genotypic salinity tolerance during long-term stress. Genotypes bearing larger numbers of tillers showed greater sensitivity to salinity due to its effects on biomass loss. Tolerant genotypes exhibited higher relative shoot biomass together with higher water use efficiency. The concentrations of Na+, K+ and their ratio, combined with the ?13C in shoots and roots were effective indicators for estimating tolerant genotypes through better water maintenance. N concentrations of shoots were the most efficient for evaluating genotypic tolerance. Low nitrate reductase (NR) and glutamine synthetase (GS) activity might be key factors limiting N accumulation. Chlorophyll (Chl) content and net photosynthetic rate, as well as stomatal conductance and evaporation, were useful for identifying salinity tolerance physiological mechanisms, but the effectiveness was low for genotypic tolerance testing for forage type oats due to the interaction between genotypes and salinity levels. The selection of high salinity-tolerant genotypes should focus on genotypes with photosynthetic resilience to salt, followed by high N metabolism (higher NR and GS activities) to ensure accumulation of more N in the shoot dry matter.

SUBMITTER: Sadaqat Shah S

PROVIDER: S-EPMC7464733 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Publications

Comparative Study of the Effects of Salinity on Growth, Gas Exchange, N Accumulation and Stable Isotope Signatures of Forage Oat (<i>Avena sativa</i> L.) Genotypes.

Sadaqat Shah Syed S Li Zhijian Z Yan Hong H Shi Lianxuan L Zhou Bangwei B

Plants (Basel, Switzerland) 20200813 8

Identifying suitable salt stress-tolerant phenotypes based on their agronomic and physiological traits remains a herculean task in forage-type oat (<i>Avena sativa</i> L.) breeding. This study examined the responses of six forage-type oat cultivars under four levels of saline stress over the vegetative growth cycle. Crop growth, water status-related traits and nitrogen status-related traits were analyzed in different plant parts to evaluate effective approaches for identifying salt tolerance. Pl ...[more]

PMID: 32823617

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Project description:BACKGROUND: Oat is an important crop in North America and northern Europe. In Scandinavia, yields are limited by the fact that oat cannot be used as a winter crop. In order to develop such a crop, more knowledge about mechanisms of cold tolerance in oat is required. RESULTS: From an oat cDNA library 9792 single-pass EST sequences were obtained. The library was prepared from pooled RNA samples isolated from leaves of four-week old Avena sativa (oat) plants incubated at +4 degrees C for 4, 8, 16 and 32 hours. Exclusion of sequences shorter than 100 bp resulted in 8508 high-quality ESTs with a mean length of 710.7 bp. Clustering and assembly identified a set of 2800 different transcripts denoted the Avena sativa cold induced UniGene set (AsCIUniGene set). Taking advantage of various tools and databases, putative functions were assigned to 1620 (58%) of these genes. Of the remaining 1180 unclassified sequences, 427 appeared to be oat-specific since they lacked any significant sequence similarity (Blast E values > 10(-10)) to any sequence available in the public databases. Of the 2800 UniGene sequences, 398 displayed significant homology (BlastX E values < or = 10(-10)) to genes previously reported to be involved in cold stress related processes. 107 novel oat transcription factors were also identified, out of which 51 were similar to genes previously shown to be cold induced. The CBF transcription factors have a major role in regulating cold acclimation. Four oat CBF sequences were found, belonging to the monocot cluster of DREB family ERF/AP2 domain proteins. Finally in the total EST sequence data (5.3 Mbp) approximately 400 potential SSRs were found, a frequency similar to what has previously been identified in Arabidopsis ESTs. CONCLUSION: The AsCIUniGene set will now be used to fabricate an oat biochip, to perform various expression studies with different oat cultivars incubated at varying temperatures, to generate molecular markers and provide tools for various genetic transformation experiments in oat. This will lead to a better understanding of the cellular biology of this important crop and will open up new ways to improve its agronomical properties.

Dataset Information

Comparative Study of the Effects of Salinity on Growth, Gas Exchange, N Accumulation and Stable Isotope Signatures of Forage Oat (Avena sativa L.) Genotypes.

Publications

Comparative Study of the Effects of Salinity on Growth, Gas Exchange, N Accumulation and Stable Isotope Signatures of Forage Oat (<i>Avena sativa</i> L.) Genotypes.

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