KIR and HLA-C genes in male infertility.

ABSTRACT: PURPOSE:Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis. METHOD:We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods. RESULTS:We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/pcorr.?=?0.0015/0.035, OR?=?1.61), KIR2DL5 gr.2 (p/pcorr.?=?0.0023/0.05, OR?=?1.64), KIR2DS2 (p/pcorr.?=?0.0019/0.044, OR?=?1.59), and KIR2DS3 (p/pcorr.?=?0.0016/0.037, OR?=?1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/pcorr.?=?0.0076/0.03, OR?=?0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/pcorr.?=?0.0076/0.03, OR?=?1.50). We also observed a limited association in KIR-HLA-C combinations. CONCLUSION:Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF.

SUBMITTER: Wilczynska K 

PROVIDER: S-EPMC7467998 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature