Dataset Information

Molecular characterization of Cachavirus firstly detected in dogs in China.

ABSTRACT: Canine Cachavirus was novel parvovirus species has been firstly identified in dogs in USA and was classified within the proposed Chaphamaparvovirus genus. To investigate Cachavirus infection in dogs in China, 408 rectal swabs from healthy and diarrheic dogs obtained during 2018-2019 were screened. The rate of Cachavirus positivity was 0% and 1.55% in healthy or diarrheic dogs, respectively. However, statistical analysis suggested no association between the presence of the virus and clinical signs (p > 0.05). Nucleotide identity was 98.2%-98.9% for NS1 and 98.6%-99.1% for VP1, and amino acid identity was 97.9%-98.7% for NS1 and 98.8%-99.6% for VP1 between the five Chinese strains and Cachavirus-1A and Cachavirus-1B detected in the United States. Phylogenetic analysis also indicated that these Cachavirus strains are genetically related to Cachavirus-1A and Cachavirus-1B. This study confirms the presence of Cachavirus in pet dogs in China and provides novel findings on its molecular characteristics.

SUBMITTER: Hu W

PROVIDER: S-EPMC7468343 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular characterization of Cachavirus firstly detected in dogs in China.

Hu Wen W Liu Qiang Q Chen Qinxi Q Ji Jun J

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20200903

Canine Cachavirus was novel parvovirus species has been firstly identified in dogs in USA and was classified within the proposed Chaphamaparvovirus genus. To investigate Cachavirus infection in dogs in China, 408 rectal swabs from healthy and diarrheic dogs obtained during 2018-2019 were screened. The rate of Cachavirus positivity was 0% and 1.55% in healthy or diarrheic dogs, respectively. However, statistical analysis suggested no association between the presence of the virus and clinical sign ...[more]

PMID: 32890765

Similar Datasets

Project description:Potato virus Y (PVY) is an important viral pathogen of the plant family Solanaceae. However, it has not been reported that PVY infects zucchini (Cucurbita pepo L). Zucchini plants showing symptoms of leaf mosaic and fruit ringspot were collected from Inner Mongolia, China. Using RT-PCR, we amplified the PVY-specific bands in zucchini (PVY-Zu isolate). Further, we found that the viral pathogen could infect Nicotiana benthamiana via mechanical inoculation with the extract of the zucchini leaves. Filamentous virions were observed in infected N. benthamiana plants under a transmission electron microscope. Additionally, the complete genomic fragment of the PVY-Zu isolate was obtained via RT-PCR and RACE analysis, containing 9662 nucleotides (nt) excluding the 3'-terminal poly (A) tail. The 5' and 3' untranslated regions (UTRs) were 150 nt and 329 nt, respectively, while the putative polyprotein contained 3061 amino acids. Comparison of the PVY-Zu isolate with 19 other PVY isolates revealed that they shared nucleotide sequence identities of ~ 83.1-99.8% at the complete genomic level. Based on the complete genome sequences, phylogenetic analysis showed that PVY-Zu clustered with PVY strain N605 (X97895-PVY), a Swiss necrotic isolate of PVY. Compared with the complete genome sequence of X97895-PVY, the PVY-Zu isolate had a deletion of a 39-nt fragment in its 5' UTR and 22 mutated nucleotides without changes in the amino acids. Moreover, although the similarity in the identities of the PVY-Zu isolate and PVY N isolate N-Jg (AY166867) was about 96.2%, the former had a deletion of a 38-nt fragment in its 5' UTR and 334 mutations among the viral genome, which resulted in 61 amino acid changes. However, we could not detect any recombination throughout the PVY-Zu genome. To our knowledge, this is the first study reporting that PVY could infect zucchini plants, thus broadening the host diversity of the viral pathogen.Supplementary informationThe online version contains supplementary material available at 10.1007/s13337-020-00647-2.

Dataset Information

Molecular characterization of Cachavirus firstly detected in dogs in China.

Publications

Molecular characterization of Cachavirus firstly detected in dogs in China.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets