Dataset Information

Insecticidal Activity of 28 Essential Oils and a Commercial Product Containing Cinnamomum cassia Bark Essential Oil against Sitophilus zeamais Motschulsky.

ABSTRACT: Maize weevils, Sitophilus zeamais, are stored product pests mostly found in warm and humid regions around the globe. In the present study, acute toxicity via contact and residual bioassay and fumigant bioassay of 28 essential oils as well as their attraction-inhibitory activity against the adults of S. zeamais were evaluated. Chemical composition of the essential oils was analyzed by gas chromatography-mass spectrometry, and a compound elimination assay was conducted on the four most active oils (cinnamon, tea tree, ylang ylang, and marjoram oils) to identify major active constituents. Amongst the oils examined, cinnamon oil was the most active in both contact/residual and fumigant bioassays, and exhibited strong behavioral inhibitory activity. Based on the compound elimination assay and chemical analyses, trans-cinnamaldehyde in cinnamon oil, and terpinen-4-ol in tea tree and marjoram oils were identified as the major active components. Although cinnamon oil seemed promising in the lab-scale bioassay without rice grains, it failed to exhibit strong insecticidal activity when the container was filled with rice. When a cinnamon oil-based product was applied both in an empty glass jar and a rice-filled container, all weevils in the empty jar were killed, whereas fewer than 15% died in the rice-filled container.

SUBMITTER: Yang Y

PROVIDER: S-EPMC7469181 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Insecticidal Activity of 28 Essential Oils and a Commercial Product Containing Cinnamomum cassia Bark Essential Oil against Sitophilus zeamais Motschulsky.

Yang Yunho Y Isman Murray B MB Tak Jun-Hyung JH

Insects 20200727 8

Maize weevils, Sitophilus zeamais, are stored product pests mostly found in warm and humid regions around the globe. In the present study, acute toxicity via contact and residual bioassay and fumigant bioassay of 28 essential oils as well as their attraction-inhibitory activity against the adults of S. zeamais were evaluated. Chemical composition of the essential oils was analyzed by gas chromatography-mass spectrometry, and a compound elimination assay was conducted on the four mo ...[more]

PMID: 32726904

Similar Datasets

Project description:BackgroundThe cereal weevil, Sitophilus zeamais is one of the most destructive pests of stored cereals worldwide. Frequent use of fumigants for managing stored-product insects has led to the development of resistance in insects. Essential oils from aromatic plants including the tea oil plant, Melaleuca alternifolia may provide environmentally friendly alternatives to currently used pest control agents. However, little is known about molecular events involved in stored-product insects in response to plant essential oil fumigation.ResultsM. alternifolia essential oil was shown to possess the fumigant toxicity against S. zeamais. The constituent, terpinen-4-ol was the most effective compound for fumigant toxicity. M. alternifolia essential oil significantly inhibited the activity of three enzymes in S. zeamais, including two detoxifying enzymes, glutathione S-transferase (GST), and carboxylesterase (CarE), as well as a nerve conduction enzyme, acetylcholinesterase (AChE). Comparative transcriptome analysis of S. zeamais through RNA-Seq identified a total of 3,562 differentially expressed genes (DEGs), of which 2,836 and 726 were up-regulated and down-regulated in response to M. alternifolia essential oil fumigation, respectively. Based on gene ontology (GO) analysis, the majority of DEGs were involved in insecticide detoxification and mitochondrial function. Furthermore, an abundance of DEGs mapped into the metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were associated with respiration and metabolism of xenobiotics, including cytochrome P450s, CarEs, GSTs, and ATP-binding cassette transporters (ABC transporters). Some DEGs mapped into the proteasome and phagosome pathway were found to be significantly enriched. These results led us to propose a model of insecticide action that M. alternifolia essential oil likely directly affects the hydrogen carrier to block the electron flow and interfere energy synthesis in mitochondrial respiratory chain.ConclusionThis is the first study to perform a comparative transcriptome analysis of S. zeamais in response to M. alternifolia essential oil fumigation. Our results provide new insights into the insecticidal mechanism of M. alternifolia essential oil fumigation against S. zeamais and eventually contribute to the management of this important agricultural pest.

Dataset Information

Insecticidal Activity of 28 Essential Oils and a Commercial Product Containing Cinnamomum cassia Bark Essential Oil against Sitophilus zeamais Motschulsky.

Publications

Insecticidal Activity of 28 Essential Oils and a Commercial Product Containing <i>Cinnamomum cassia</i> Bark Essential Oil against <i>Sitophilus zeamais</i> Motschulsky.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets