Dataset Information

Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions.

ABSTRACT: Bees collect pollen from flowers for their offspring, and by doing so contribute critical pollination services for our crops and ecosystems. Unlike many managed bee species, wild bees are thought to obtain much of their microbiome from the environment. However, we know surprisingly little about what plant species bees visit and the microbes associated with the collected pollen. Here, we addressed the hypothesis that the pollen and microbial components of bee diets would change across the range of the bee, by amplicon sequencing pollen provisions of a widespread small carpenter bee, Ceratina calcarata, across three populations. Ceratina calcarata was found to use a diversity of floral resources across its range, but the bacterial genera associated with pollen provisions were very consistent. Acinetobacter, Erwinia, Lactobacillus, Sodalis, Sphingomonas and Wolbachia were among the top ten bacterial genera across all sites. Ceratina calcarata uses both raspberry (Rubus) and sumac (Rhus) stems as nesting substrates, however nests within these plants showed no preference for host plant pollen. Significant correlations in plant and bacterial co-occurrence differed between sites, indicating that many of the most common bacterial genera have either regional or transitory floral associations. This range-wide study suggests microbes present in brood provisions are conserved within a bee species, rather than mediated by climate or pollen composition. Moving forward, this has important implications for how these core bacteria affect larval health and whether these functions vary across space and diet. These data increase our understanding of how pollinators interact with and adjust to their changing environment.

SUBMITTER: Dew RM

PROVIDER: S-EPMC7469187 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions.

Dew Rebecca M RM McFrederick Quinn S QS Rehan Sandra M SM

Insects 20200804 8

Bees collect pollen from flowers for their offspring, and by doing so contribute critical pollination services for our crops and ecosystems. Unlike many managed bee species, wild bees are thought to obtain much of their microbiome from the environment. However, we know surprisingly little about what plant species bees visit and the microbes associated with the collected pollen. Here, we addressed the hypothesis that the pollen and microbial components of bee diets would change across the range o ...[more]

PMID: 32759653

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Project description:Primates have evolved a variety of feeding habits and intestinal physiological structure. Gut microbiome act as metabolic organs in many biological processes and play a vital role in adaptation to dietary niches. Gut microbiome also convert primary bile acids (BAs) to secondary. BAs profile and gut microbiome are together influenced by diets and play a significant role in nutrient absorption. The regulation between gut microbiome and BAs metabolism is bidirectional although the relationship in primates consuming diverse diets is still unclear. Here, we investigated gut microbiome structures, fecal BAs profile, and their relationship in primates preferring three distinct diets. We found that gut microbiome communities are well differentiated among dietary groups. Folivorous primates had higher Firmicutes abundance and lower Prevotella to Bacaeroides ratios, possibly related to fiber consumption. Frugivorous primates are colonized predominantly by Prevotella and Bacteroides, pointing to an increased adaptation to high-sugar and simple carbohydrate diets. Likewise, BA profiles differ according to diet in a manner predictable from the known effects of BAs on metabolism. Folivorous primates have high conjugated bile acid levels and low unconjugated to conjugated BA ratios, consistent with their fiber-rich leaf-eating diet. Much of the differentiation in secondary and unconjugated BAs is associated with microbiome composition shifts and individual bile acid concentrations are correlated with the abundance of distinct bacterial taxonomic groups. Omnivores have higher concentrations of secondary BAs, mainly lithocholic acid (LCA). These levels are significantly positively correlated with the presence of Clostrida species, showing that the digestion requirements of omnivores are different from plant-eating primates. In conclusion, gut microbiome and BAs can respond to changes in diet and are associated with nutrient component consumption in each diet primate group. Our study is the first to demonstrate BA profile differentiation among primates preferring diverse diets. BAs thus appear to work with gut microbiome to help primates adapt to their diet.

Project description:Simple Summary Broiler meat provides a considerable nutritional source of animal protein in the human diet. However, the intensive and accelerating growth in broiler breeding programs requires a continuous or intermittent use of antibiotics to improve the health and performance of broilers. Antibiotic resistance and residues problems cause a general limitation on the use of antibiotics in poultry production worldwide, and hence, prompt researchers and feed companies to find natural alternatives. In the present study, we investigated the possible impact of propolis (PR) and bee pollen (BP) in nutritional strategies on the performance and immunity of broiler chickens. The obtained results display the ability of PR and BP contained within the broiler diets to enhance the antioxidant defense system and improve several immunological parameters. These beneficial effects coincided with an increase in the growth performance of broilers. Thus, supplementation of PR and BP separately or in combination could be recommended into broiler diets for their positive impacts as natural products on the performance and health of broilers. Abstract (1) Background: Propolis and bee pollen have natural bioactive compounds that may support the performance and immunological response of broilers. (2) Methods: The study included 300 1 d old Cobb-500 broiler chicks. Starting from 22–42 d of age, chicks were divided according to a 2 × 2 factorial design into one of the four treatment groups (5 replicates × 15 chicks per replicate); a basal diet without supplementation (CONT) or supplemented with 1 g/kg of propolis (PR) or bee pollen (BP) separately or in an even combination (PR + BP). (3) Results: A significant (p < 0.05) increase was obtained in the body-weight gain of broilers treated with PR, BP, and PR + BP compared to the CONT. The total antioxidant capacity and superoxide dismutase were highly (p < 0.05) activated in all treated groups compared to the CONT. Immunological parameters, especially the leukocyte cell viability, T- and B-lymphocyte proliferation, immunoglobulins (IgA and IgM), antibody titers, and wattle-swelling test were significantly (p < 0.05) enhanced in the treated broilers with PR and/or BP compared to the CONT. (4) Conclusions: The dietary supplementation of PR and/or BP could be beneficial for broiler growth through maximizing the antioxidant- and immune-system defenses.

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Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions.

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Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions.

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