Binding of 2-(Triazolylthio)acetamides to Metallo-?-lactamase CcrA Determined with NMR.

ABSTRACT: Metallo-?-lactamase (MBL)-producing bacteria resistant to ?-lactam antibiotics are a serious threat to human health. Despite great efforts and important progress in the discovery of MBL inhibitors (MBLIs), there is none in clinical use. Herein, inhibitor complexes of the MBL CcrA were investigated by NMR spectroscopy to provide perspectives on the further development of 2-(triazolylthio)acetamide-type MBLIs. By using the NMR-based chemical shift perturbation (CSP) and direction of CSP methodologies together with molecular docking, the spatial orientation of three compounds in the CcrA active site was investigated (4-6). Inhibitor 6 showed the best binding affinity (K d ? 2.3 ± 0.3 ?M), followed by 4 (K d = 11 ± 11 ?M) and 5 (K d = 34 ± 43 ?M), as determined from the experimental NMR data. Based on the acquired knowledge, analogues of other MBLIs (1-3) were designed and evaluated in silico with the purpose of examining a strategy for promoting their interactions with the catalytic zinc ions.

SUBMITTER: Andersson H 

PROVIDER: S-EPMC7469393 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature