Ontology highlight
ABSTRACT:
SUBMITTER: Fry LE
PROVIDER: S-EPMC7469699 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Fry Lewis E LE Peddle Caroline F CF Stevanovic Marta M Barnard Alun R AR McClements Michelle E ME MacLaren Robert E RE
The CRISPR journal 20200801 4
Adeno-associated virus (AAV) vectors have been widely adopted for delivery of CRISPR-Cas components, especially for therapeutic gene editing. For a single vector system, both the Cas9 and guide RNA (gRNA) are encoded within a single transgene, usually from separate promoters. Careful design of this bi-cistronic construct is required due to the minimal packaging capacity of AAV. We investigated how placement of the U6 promoter expressing the gRNA on the reverse strand to SaCas9 driven by a cytome ...[more]