Unknown

Dataset Information

0

Temperature-gradient incubation isolates multiple competitive species from a single environmental sample.


ABSTRACT: High-throughput sequencing has allowed culture-independent investigation into a wide variety of microbiomes, but sequencing studies still require axenic culture experiments to determine ecological roles, confirm functional predictions and identify useful compounds and pathways. We have developed a new method for culturing and isolating multiple microbial species with overlapping ecological niches from a single environmental sample, using temperature-gradient incubation. This method was more effective than standard serial dilution-to-extinction at isolating methanotrophic bacteria. It also highlighted discrepancies between culture-dependent and -independent techniques; 16S rRNA gene amplicon sequencing of the same sample did not accurately reflect cultivatable strains using this method. We propose that temperature-gradient incubation could be used to separate out and study previously 'unculturable' strains, which co-exist in both natural and artificial environments.

SUBMITTER: Houghton KM 

PROVIDER: S-EPMC7470311 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| PRJNA419899 | ENA
| S-EPMC9826120 | biostudies-literature
| S-EPMC2075052 | biostudies-literature
| S-EPMC4904169 | biostudies-literature
| S-EPMC9355520 | biostudies-literature
| PRJNA561052 | ENA
| S-EPMC4278824 | biostudies-literature
| S-EPMC8845567 | biostudies-literature
| S-EPMC11005360 | biostudies-literature
2023-10-01 | GSE121165 | GEO