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Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies.


ABSTRACT: A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and Ixodes ricinus tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

SUBMITTER: Helmova R 

PROVIDER: S-EPMC7472235 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies.

Helmová Renata R   Hönig Václav V   Tykalová Hana H   Palus Martin M   Bell-Sakyi Lesley L   Grubhoffer Libor L  

Viruses 20200818 8


A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and <i>Ixodes ricinus</i> tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exi  ...[more]

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