Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability.
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ABSTRACT:
SUBMITTER: Gomes FP
PROVIDER: S-EPMC7474054 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
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Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability.
Gomes Fabio P FP Park Robin R Viana Arabela G AG Fernandez-Costa Carolina C Topper Einko E Kaya Abdullah A Memili Erdogan E Yates John R JR Moura Arlindo A AA
Scientific reports 20200904 1
The present study investigated the seminal plasma proteome of Holstein bulls with low (LF; n = 6) and high (HF; n = 8) sperm freezability. The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry varied from -2.2 in LF to + 7.8 in HF bulls, as compared to the average %ViableSperm (54.7%) measured in an 860-sire population. Seminal proteins were analyzed by label free mass spectrometry, with the support of statistical and bioinformatics analyses. This approach iden ...[more]
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