Ontology highlight
ABSTRACT:
SUBMITTER: Choi WS
PROVIDER: S-EPMC7474658 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Choi Woo Suk WS He Peng P Pothukuchy Arti A Gollihar Jimmy J Ellington Andrew D AD Yang Wei W
Proceedings of the National Academy of Sciences of the United States of America 20200817 35
We report here crystal structures of a reverse transcriptase RTX, which was evolved in vitro from the B family polymerase KOD, in complex with either a DNA duplex or an RNA-DNA hybrid. Compared with the apo, binary, and ternary complex structures of the original KOD polymerase, the 16 substitutions that result in the function of copying RNA to DNA do not change the overall protein structure. Only six substitutions occur at the substrate-binding surface, and the others change domain-domain interf ...[more]