Dataset Information

Genetic variability analysis of partially salt tolerant local and inbred rice (Oryza sativa L.) through molecular markers.

ABSTRACT: Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic variability in sixteen selected germplasms of rice, Oryza sativa L. using eight decamer RAPD primers. The data obtained from this investigation reveals a high level of polymorphism between cultivars. The primers produced a total of 255 bands of which all 255 bands were polymorphic indicating 100% polymorphism. The size of the amplified bands ranged from 220 bp to 2290 bp. The number of polymorphic fragments ranged from 24 to 49 with an average of 32 polymorphic fragments for each primer. The primer OPX04 produced the maximum number (49) of polymorphic bands while the OPB04 and OPB17 produced the minimum number (24) of polymorphic bands. The polymorphic information content (PIC) values ranged from 0.6616 to 0.8845 with an average of 0.832. The highest PIC value (0.8845) was obtained for primer OPL03. The RAPD data was analyzed to determine the pair-wise genetic similarity coefficients which ranged from 0.00 to 0.83. The BRRIdhan 23 and the BRRIdhan 41 varieties were the closest genotypes with the highest similarity index of 83%. This was followed by 77% similarity between a pair of cultivars Kalamona and Horkuch. On the other hand, 100% dissimilarity was seen between BRRIdhan 53, BRRIdhan 50, BRRIdhan 10, BRRIdhan 70, BRRIdhan 54, BRRIdhan 40, BRRIdhan 23, BRRIdhan 47, BRRIdhan 41 and Dadsail respectively and between BRRIdhan 53 and Horkuch; indicating a high level of variability between paired genotypes. Cluster analysis was performed using Unweighted Paired Group of Arithmetic Means (UPGMA). The UPGMA dendrogram resolved the selected rice cultivars into four clusters.

SUBMITTER: Mazumder SR

PROVIDER: S-EPMC7475118 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Genetic variability analysis of partially salt tolerant local and inbred rice (<i>Oryza sativa</i> L.) through molecular markers.

Mazumder Sonia Rani SR Hoque Hammadul H Sinha Beethi B Chowdhury Woasifur Rahman WR Hasan Md Nazmul MN Prodhan Shamsul H SH

Heliyon 20200826 8

Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic variability in sixteen selected germplasms of rice, <i>Oryza sativa</i> L. using eight decamer RAPD primers. The data obtained from this investigation reveals a high level of polymorphism between cultivars. The primers produced a total of 255 bands of which all 255 bands were polymorphic indicating 100% polymorphism. The size of the amplified bands ranged from 220 bp to 2290 bp. The number of polymorphic fragmen ...[more]

PMID: 32923704

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Dataset Information

Genetic variability analysis of partially salt tolerant local and inbred rice (Oryza sativa L.) through molecular markers.

Publications

Genetic variability analysis of partially salt tolerant local and inbred rice (<i>Oryza sativa</i> L.) through molecular markers.

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