Ontology highlight
ABSTRACT:
SUBMITTER: Hollandsworth HM
PROVIDER: S-EPMC7475271 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Hollandsworth Hannah M HM Schmitt Verena V Amirfakhri Siamak S Filemoni Filemoni F Schmidt Alexej A Landström Maréne M Lyndin Mykola M Backert Steffen S Gerhard Markus M Wennemuth Gunther G Hoffman Robert M RM Singer Bernhard B BB Bouvet Michael M
Translational oncology 20200828 12
HopQ is an outer-membrane protein of Helicobacter pylori that binds to human carcinoembryonic antigen-related cell-adhesion molecules (CEACAMs) with high specificity. We aimed to investigate fluorescence targeting of CEACAM-expressing colorectal tumors in patient-derived orthotopic xenograft (PDOX) models with fluorescently labeled recombinant HopQ (rHopQ). Western blotting, flow cytometry and ELISA were performed to determine the efficiency of rHopQ binding to CEACAMs. rHopQ was conjugated to I ...[more]