Underground isoleucine biosynthesis pathways in E. coli.
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ABSTRACT:
SUBMITTER: Cotton CA
PROVIDER: S-EPMC7476758 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
Underground isoleucine biosynthesis pathways in <i>E. coli</i>.
Cotton Charles Ar CA Bernhardsgrütter Iria I He Hai H Burgener Simon S Schulz Luca L Paczia Nicole N Dronsella Beau B Erban Alexander A Toman Stepan S Dempfle Marian M De Maria Alberto A Kopka Joachim J Lindner Steffen N SN Erb Tobias J TJ Bar-Even Arren A
eLife 20200824
The promiscuous activities of enzymes provide fertile ground for the evolution of new metabolic pathways. Here, we systematically explore the ability of <i>E. coli</i> to harness underground metabolism to compensate for the deletion of an essential biosynthetic pathway. By deleting all threonine deaminases, we generated a strain in which isoleucine biosynthesis was interrupted at the level of 2-ketobutyrate. Incubation of this strain under aerobic conditions resulted in the emergence of a novel ...[more]
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