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Sensitive and Selective Measurement of Serotonin in Vivo Using Fast Cyclic Square-Wave Voltammetry.


ABSTRACT: Although N-shaped fast scan cyclic voltammetry (N-FSCV) is well-established as an electroanalytical method to measure extracellular serotonin concentrations in vivo, it is in need of improvement in both sensitivity and selectivity. Based on our previous studies using fast cyclic square-wave voltammetry (FCSWV) for in vivo dopamine measurements, we have modified this technique to optimize the detection of serotonin in vivo. A series of large amplitude square-shaped potentials was superimposed onto an N-shaped waveform to provide cycling through multiple redox reactions within the N-shaped waveform to enhance the sensitivity and selectivity to serotonin measurement when combined with a two-dimensional voltammogram. N-Shaped fast cyclic square-wave voltammetry (N-FCSWV) showed significantly higher sensitivity to serotonin compared to conventional N-FSCV. In addition, N-FCSWV showed better performance than conventional N-shaped FSCV in differentiating serotonin from its major interferents, dopamine and 5-hydroxyindoleascetic acid (5-HIAA). It was also confirmed that the large amplitude of the square waveform did not influence local neuronal activity, and it could monitor electrical stimulation evoked phasic release of serotonin in the rat substantia nigra pars reticulata (SNr) before and after systemic injection of escitalopram (ESCIT, 10 mg/kg i.p.), a serotonin selective reuptake inhibitor.

SUBMITTER: Shin H 

PROVIDER: S-EPMC7478172 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Sensitive and Selective Measurement of Serotonin <i>in Vivo</i> Using Fast Cyclic Square-Wave Voltammetry.

Shin Hojin H   Oh Yoonbae Y   Park Cheonho C   Kang Yumin Y   Cho Hyun U HU   Blaha Charles D CD   Bennet Kevin E KE   Heien Michael L ML   Kim In Young IY   Lee Kendall H KH   Jang Dong Pyo DP  

Analytical chemistry 20191216 1


Although N-shaped fast scan cyclic voltammetry (N-FSCV) is well-established as an electroanalytical method to measure extracellular serotonin concentrations <i>in vivo</i>, it is in need of improvement in both sensitivity and selectivity. Based on our previous studies using fast cyclic square-wave voltammetry (FCSWV) for <i>in vivo</i> dopamine measurements, we have modified this technique to optimize the detection of serotonin <i>in vivo</i>. A series of large amplitude square-shaped potentials  ...[more]

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