Dataset Information

Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability.

ABSTRACT: Nitrogen fertilization is common for poplar trees to improve growth and productivity. The utilization of N by poplar largely depends on fertilizer application patterns; however, the underlying regulatory hubs are not fully understood. In this study, N utilization and potentially physiological regulations of two poplar clones (XQH and BC5) were assessed through two related experiments (i: five levels of N supply and ii: conventional and exponential N additions). Poplar growth (leaf area) and N utilization significantly increased under fertilized compared to unfertilized conditions, whereas photosynthetic N utilization efficiency significantly decreased under low N supplies. Growth characteristics were better in the XQH than in the BC5 clone under the same N supplies, indicating higher N utilization efficiency. Leaf absorbed light energy, and thermal dissipation fraction was significantly different for XQH clone between conventional and exponential N additions. Leaf concentrations of putrescine (Put) and acetylated Put were significantly higher in exponential than in conventional N addition. Photorespiration significantly increased in leaves of XQH clone under exponential compared to conventional N addition. Our results indicate that an interaction of the clone and N supply pattern significantly occurs in poplar growth; leaf expansion and the storage N allocations are the central hubs in the regulation of poplar N utilization.

SUBMITTER: Hu Y

PROVIDER: S-EPMC7479266 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability.

Hu Yanbo Y Siddiqui Manzer H MH Li Chunming C Jiang Luping L Zhang Heng H Zhao Xiyang X

Frontiers in plant science 20200826

Nitrogen fertilization is common for poplar trees to improve growth and productivity. The utilization of N by poplar largely depends on fertilizer application patterns; however, the underlying regulatory hubs are not fully understood. In this study, N utilization and potentially physiological regulations of two poplar clones (XQH and BC5) were assessed through two related experiments (i: five levels of N supply and ii: conventional and exponential N additions). Poplar growth (leaf area) and N ut ...[more]

PMID: 32983189

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Project description:Photosystem II (PSII) is a multisubunit pigment-protein complex that uses light-induced charge separation to power oxygenic photosynthesis. Its reaction center chromophores, where the charge transfer cascade is initiated, are arranged symmetrically along the D1 and D2 core polypeptides and comprise four chlorophyll (PD1, PD2, ChlD1, ChlD2) and two pheophytin molecules (PheoD1 and PheoD2). Evolution favored productive electron transfer only via the D1 branch, with the precise nature of primary excitation and the factors that control asymmetric charge transfer remaining under investigation. Here we present a detailed atomistic description for both. We combine large-scale simulations of membrane-embedded PSII with high-level quantum-mechanics/molecular-mechanics (QM/MM) calculations of individual and coupled reaction center chromophores to describe reaction center excited states. We employ both range-separated time-dependent density functional theory and the recently developed domain based local pair natural orbital (DLPNO) implementation of the similarity transformed equation of motion coupled cluster theory with single and double excitations (STEOM-CCSD), the first coupled cluster QM/MM calculations of the reaction center. We find that the protein matrix is exclusively responsible for both transverse (chlorophylls versus pheophytins) and lateral (D1 versus D2 branch) excitation asymmetry, making ChlD1 the chromophore with the lowest site energy. Multipigment calculations show that the protein matrix renders the ChlD1 → PheoD1 charge-transfer the lowest energy excitation globally within the reaction center, lower than any pigment-centered local excitation. Remarkably, no low-energy charge transfer states are located within the "special pair" PD1-PD2, which is therefore excluded as the site of initial charge separation in PSII. Finally, molecular dynamics simulations suggest that modulation of the electrostatic environment due to protein conformational flexibility enables direct excitation of low-lying charge transfer states by far-red light.

Dataset Information

Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability.

Publications

Polyamine Metabolism, Photorespiration, and Excitation Energy Allocation in Photosystem II Are Potentially Regulatory Hubs in Poplar Adaptation to Soil Nitrogen Availability.

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