Ontology highlight
ABSTRACT:
SUBMITTER: Dopie J
PROVIDER: S-EPMC7480118 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Dopie Joseph J Sweredoski Michael J MJ Moradian Annie A Belmont Andrew S AS
The Journal of cell biology 20200901 9
We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in nuclear speckles versus centromeres. Our "TSA-MS ratio" approach successfully identified known nuclear speckle proteins. For example, 96% and 67% of proteins in the top 30 and 100 sorted proteins, respectively, are kn ...[more]