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Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma.

ABSTRACT: A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.

SUBMITTER: Farrera-Soler L

PROVIDER: S-EPMC7480855 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma.

Farrera-Soler Lluc L Daguer Jean-Pierre JP Barluenga Sofia S Vadas Oscar O Cohen Patrick P Pagano Sabrina S Yerly Sabine S Kaiser Laurent L Vuilleumier Nicolas N Winssinger Nicolas N

PloS one 20200909 9

A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evi ...[more]

PMID: 32903266

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