Genetic variation contributes to gene expression response in ischemic stroke: an eQTL study.

ABSTRACT:

Objective

Single nucleotide polymorphisms (SNPs) contribute to complex disorders such as ischemic stroke (IS). Since SNPs could affect IS by altering gene expression, we studied the association of common SNPs with changes in mRNA expression (i.e. expression quantitative trait loci; eQTL) in blood after IS.

Methods

RNA and DNA were isolated from 137 patients with acute IS and 138 vascular risk factor controls (VRFC). Gene expression was measured using Affymetrix HTA 2.0 microarrays and SNP variants were assessed with Axiom Biobank Genotyping microarrays. A linear model with a genotype (SNP) × diagnosis (IS and VRFC) interaction term was fit for each SNP-gene pair.

Results

The eQTL interaction analysis revealed significant genotype × diagnosis interaction for four SNP-g

SUBMITTER: Amini H 

PROVIDER: S-EPMC7480928 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature