Ontology highlight
ABSTRACT:
SUBMITTER: Abdullah
PROVIDER: S-EPMC7481536 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Abdullah Jiang Zhengzheng Z Hong Xulin X Zhang Shun S Yao Ruilian R Xiao Yi Y
Synthetic and systems biotechnology 20200902 4
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing. The RNA guided Cas nucleases generate DNA double-strand break (DSB), triggering cellular repair systems mainly Non-homologous end-joining (NHEJ, imprecise repair) or Homology-directed repair (HDR, precise repair). However, DSB typically leads to unexpected DNA changes and lethality in some organisms. The establishment of bacteria and plants ...[more]