Dataset Information

Draft Genome Sequence of Streptococcus thermophilus Strain CBC-S77, Isolated from Homemade Dairy Foods in Bulgaria.

ABSTRACT: Here, we report the draft genome sequence of Streptococcus thermophilus strain CBC-S77. The strain was originally isolated from naturally processed, homemade dairy foods in West Rhode Mountain, Bulgaria. The genome was assembled in 148 contigs with a total length of 1,707,130?bp, with 1,563 coding genes and a GC content of 39.11%.

SUBMITTER: Shehata HR

PROVIDER: S-EPMC7484077 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Draft Genome Sequence of Streptococcus thermophilus Strain CBC-S77, Isolated from Homemade Dairy Foods in Bulgaria.

Shehata Hanan R HR Chandler Richmond A RA Newmaster Steven G SG

Microbiology resource announcements 20200910 37

Here, we report the draft genome sequence of <i>Streptococcus thermophilus</i> strain CBC-S77. The strain was originally isolated from naturally processed, homemade dairy foods in West Rhode Mountain, Bulgaria. The genome was assembled in 148 contigs with a total length of 1,707,130 bp, with 1,563 coding genes and a GC content of 39.11%. ...[more]

PMID: 32912918

Similar Datasets

Project description:BackgroundStreptococcus thermophilus is a major dairy starter used for manufacturing of dairy products. In the present study, we developed a multilocus sequence typing (MLST) scheme for this important food bacterium. Sequences of 10 housekeeping genes (carB, clpX, dnaA, murC, murE, pepN, pepX, pyrG, recA, and rpoB) were obtained for 239 S. thermophilus strains, which were isolated from home-made fermented dairy foods in 18 different regions of Mongolia and China.MethodsAll 10 genes of S. thermophilus were sequenced, aligned, and defined sequence types (STs) using the BioNumerics Software. The nucleotide diversity was calculated by START v2.0. The population structure, phylogenetic relationships and the role of recombination were inferred using ClonalFrame v1.2, SplitsTree 4.0 and Structure v2.3.ResultsThe 239 S. thermophilus isolates and 18 reference strains could be assigned into 119 different STs, which could be further separated into 16 clonal complexes (CCs) and 38 singletons. Among the 10 loci, a total of 132 polymorphic sites were detected. The standardized index of association (IAS=0.0916), split-decomposition and ρ/θ (relative frequency of occurrence of recombination and mutation) and r/m value (relative impact of recombination and mutation in the diversification) confirms that recombination may have occurred, but it occurred at a low frequency in these 10 loci. Phylogenetic trees indicated that there were five lineages in the S. thermophilus isolates used in our study. MSTree and ClonalFrame tree analyses suggest that the evolution of S. thermophilus isolates have little relationship with geographic locality, but revealed no association with the types of fermented dairy product. Phylogenetic analysis of 36 whole genome strains (18 S. thermophilus, 2 S. vestibularis and 16 S. salivarius strains) indicated that our MLST scheme could clearly separate three closely related species within the salivarius group and is suitable for analyzing the population structure of the other two species in the salivarius group.ConclusionsOur newly developed MLST scheme improved the understanding on the genetic diversity and population structure of the S. thermophilus, as well as provided useful information for further studies on the genotyping and evolutionary research for S. thermophilus strains with global diversity.

Dataset Information

Draft Genome Sequence of Streptococcus thermophilus Strain CBC-S77, Isolated from Homemade Dairy Foods in Bulgaria.

Publications

Draft Genome Sequence of Streptococcus thermophilus Strain CBC-S77, Isolated from Homemade Dairy Foods in Bulgaria.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets