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Monitoring protein-metal binding by ¹⁹F NMR - a case study with the New Delhi metallo-?-lactamase 1.

ABSTRACT: ¹⁹F NMR protein observed spectroscopy is evaluated as a method for analysing protein metal binding using the New Delhi metallo-?-lactamase 1. The results imply ¹⁹F NMR is useful for analysis of different metallated protein states and investigations on equilibrium states in the presence of inhibitors. One limitation is that ¹⁹F labelling may affect metal ion binding. The sensitive readout of changes in protein behaviour observed by ¹⁹F NMR spectra coupled with the broad scope of tolerated conditions (e.g. buffer variations) means ¹⁹F NMR should be further investigated for studying metal ion interactions and the inhibition of metallo-enzymes during drug discovery.

SUBMITTER: Rydzik AM

PROVIDER: S-EPMC7484990 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Monitoring protein-metal binding by 19F NMR - a case study with the New Delhi metallo-β-lactamase 1.

Rydzik Anna M AM Brem Jürgen J Chandler Shane A SA Benesch Justin L P JLP Claridge Timothy D W TDW Schofield Christopher J CJ

RSC medicinal chemistry 20200221 3

19F NMR protein observed spectroscopy is evaluated as a method for analysing protein metal binding using the New Delhi metallo-β-lactamase 1. The results imply 19F NMR is useful for analysis of different metallated protein states and investigations on equilibrium states in the presence of inhibitors. One limitation is that 19F labelling may affect metal ion binding. The sensitive readout of changes in protein behaviour observed by 19F NMR spectra coupl ...[more]

PMID: 33479644

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