Dataset Information

Inflammatory Activity in Atelectatic and Normally Aerated Regions During Early Acute Lung Injury.

ABSTRACT:

Rationale and objectives

Pulmonary atelectasis presumably promotes and facilitates lung injury. However, data are limited on its direct and remote relation to inflammation. We aimed to assess regional 2-deoxy-2-[¹⁸F]-fluoro-D-glucose (¹⁸F-FDG) kinetics representative of inflammation in atelectatic and normally aerated regions in models of early lung injury.

Materials and methods

We studied supine sheep in four groups: Permissive Atelectasis (n = 6)-16 hours protective tidal volume (V_T) and zero positive end-expiratory pressure; Mild (n = 5) and Moderate Endotoxemia (n = 6)- 20-24 hours protective ventilation and intravenous lipopolysaccharide (Mild = 2.5 and Moderate = 10.0 ng/kg/min), and Surfactant Depletion (n = 6)-saline lung lavage and 4 hours high V_T. Measurements performed immediately after anesthesia induction served as controls (n = 8). Atelectasis was defined as regions of gas fraction <0.1 in transmission or computed tomography scans. ¹⁸F-FDG kinetics measured with positron emission tomography were analyzed with a three-compartment model.

Results

¹⁸F-FDG net uptake rate in atelectatic tissue was larger during Moderate Endotoxemia (0.0092 ± 0.0019/min) than controls (0.0051 ± 0.0014/min, p = 0.01). ¹⁸F-FDG phosphorylation rate in atelectatic tissue was larger in both endotoxemia groups (0.0287 ± 0.0075/min) than controls (0.0198 ± 0.0039/min, p = 0.05) while the ¹⁸F-FDG volume of distribution was not significantly different among groups. Additionally, normally aerated regions showed larger ¹⁸F-FDG uptake during Permissive Atelectasis (0.0031 ± 0.0005/min, p < 0.01), Mild (0.0028 ± 0.0006/min, p = 0.04), and Moderate Endotoxemia (0.0039 ± 0.0005/min, p < 0.01) than controls (0.0020 ± 0.0003/min).

Conclusion

Atelectatic regions present increased metabolic activation during moderate endotoxemia mostly due to increased ¹⁸F-FDG phosphorylation, indicative of increased cellular metabolic activation. Increased ¹⁸F-FDG uptake in normally aerated regions during permissive atelectasis suggests an injurious remote effect of atelectasis even with protective tidal volumes.

SUBMITTER: Hinoshita T

PROVIDER: S-EPMC7486228 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

Inflammatory Activity in Atelectatic and Normally Aerated Regions During Early Acute Lung Injury.

Hinoshita Takuga T Ribeiro Gabriel Motta GM Winkler Tilo T de Prost Nicolas N Tucci Mauro R MR Costa Eduardo Leite Vieira ELV Wellman Tyler J TJ Hashimoto Soshi S Zeng Congli C Carvalho Alysson R AR Melo Marcos Francisco Vidal MFV

Academic radiology 20200312 12

<h4>Rationale and objectives</h4>Pulmonary atelectasis presumably promotes and facilitates lung injury. However, data are limited on its direct and remote relation to inflammation. We aimed to assess regional 2-deoxy-2-[<sup>18</sup>F]-fluoro-D-glucose (<sup>18</sup>F-FDG) kinetics representative of inflammation in atelectatic and normally aerated regions in models of early lung injury.<h4>Materials and methods</h4>We studied supine sheep in four groups: Permissive Atelectasis (n = 6)-16 hours p ...[more]

PMID: 32173290

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Project description:Normally aerated lung tissue on computed tomography (CT) is correlated with static respiratory system compliance (Crs) at zero end-expiratory pressure. In clinical practice, however, patients with acute respiratory failure are often managed using elevated PEEP levels. No study has validated the relationship between lung volume and tissue and Crs at the applied positive end-expiratory pressure (PEEP). Therefore, this study aimed to demonstrate the relationship between lung volume and tissue on CT and Crs during the application of PEEP for the clinical management of patients with acute respiratory distress syndrome due to COVID-19. Additionally, as a secondary outcome, the study aimed to evaluate the relationship between CT characteristics and Crs, considering recruitability using the recruitment-to-inflation ratio (R/I ratio). We analyzed the CT and respiratory mechanics data of 30 patients with COVID-19 who were mechanically ventilated. The CT images were acquired during mechanical ventilation at PEEP level of 15 cmH2O and were quantitatively analyzed using Synapse Vincent system version 6.4 (Fujifilm Corporation, Tokyo, Japan). Recruitability was stratified into two groups, high and low recruitability, based on the median R/I ratio of our study population. Thirty patients were included in the analysis with the median R/I ratio of 0.71. A significant correlation was observed between Crs at the applied PEEP (median 15 [interquartile range (IQR) 12.2, 15.8]) and the normally aerated lung volume (r = 0.70 [95% CI 0.46-0.85], P < 0.001) and tissue (r = 0.70 [95% CI 0.46-0.85], P < 0.001). Multivariable linear regression revealed that recruitability (Coefficient = - 390.9 [95% CI - 725.0 to - 56.8], P = 0.024) and Crs (Coefficient = 48.9 [95% CI 32.6-65.2], P < 0.001) were significantly associated with normally aerated lung volume (R-squared: 0.58). In this study, Crs at the applied PEEP was significantly correlated with normally aerated lung volume and tissue on CT. Moreover, recruitability indicated by the R/I ratio and Crs were significantly associated with the normally aerated lung volume. This research underscores the significance of Crs at the applied PEEP as a bedside-measurable parameter and sheds new light on the link between recruitability and normally aerated lung.

Dataset Information

Inflammatory Activity in Atelectatic and Normally Aerated Regions During Early Acute Lung Injury.

Rationale and objectives

Materials and methods

Results

Conclusion

Publications

Inflammatory Activity in Atelectatic and Normally Aerated Regions During Early Acute Lung Injury.

Similar Datasets

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