LncRNA THRIL is upregulated in sepsis and sponges miR-19a to upregulate TNF-? in human bronchial epithelial cells.

ABSTRACT: Background:Long non-coding RNAs (lncRNAs) have been demonstrated to play critical roles in various diseases. Our bioinformatics analysis showed that lncRNA TNF? and heterogenous nuclear ribonucleoprotein L (hnRNPL) related immunoregulatory LincRNA (THRIL) may interact with miR-19a, which targets TNF-?. This study aimed to explore the role of THRIL, an enhancer of LPS-induced inflammatory, in sepsis. Methods:Research subjects of the present study included 66 sepsis patients and 66 healthy volunteers. The expression levels of THRIL, miR-19a and TNF-? in plasma samples from these participants were determined by RT-qPCR. The interaction between THRIL and miR-19a was explored by performing overexpression experiments in human bronchial epithelial cells (HBEpCs). The roles of THRIL, miR-19a and TNF-? in regulating the apoptosis of HBEpCs were analyzed by cell apoptosis assay. Results:We found that THRIL was upregulated in sepsis patients. THRIL is predicted to interact with miR-19a, and the interaction was confirmed by dual-luciferase activity assay. However, THRIL and miR-19a did not affect the expression of each other. Instead, overexpression of THRIL resulted in the increased expression levels of TNF-?, a downstream target of miR-19a in HBEpCs. In HBEpCs, LPS treatment induced the overexpression of THRIL. Cell apoptosis analysis showed that overexpression of THRIL and TNF-? promoted the apoptosis of HBEpCs induced by LPS, while overexpression of miR-19a played an opposite role. Overexpression of THRIL attenuated the effects of overexpression of miR-19a. Conclusion:Therefore, THRIL is upregulated in sepsis and may sponge miR-19a to upregulate TNF-?, thereby promoting lung cell apoptosis.

SUBMITTER: Liu T 

PROVIDER: S-EPMC7488348 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature