Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion.

ABSTRACT:

Background

The quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved methodologies for quantification of CNMs in vital organs is instrumental in their continued modification and application.

Results

In this study, carbon black, nanodiamond, multi-walled carbon nanotube, carbon nanofiber, and graphene nanoplatelet were assembled and used as a panel of CNMs. All CNMs showed significant absorbance at 750 nm, while their bio-components showed minimal absorbance at this wavelength. Quantification of CNMs using their absorbance at 750 nm was shown to have more

SUBMITTER: Lee DK 

PROVIDER: S-EPMC7488454 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature