Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies.
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ABSTRACT:
SUBMITTER: Rhie A
PROVIDER: S-EPMC7488777 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies.
Rhie Arang A Walenz Brian P BP Koren Sergey S Phillippy Adam M AM
Genome biology 20200914 1
Recent long-read assemblies often exceed the quality and completeness of available reference genomes, making validation challenging. Here we present Merqury, a novel tool for reference-free assembly evaluation based on efficient k-mer set operations. By comparing k-mers in a de novo assembly to those found in unassembled high-accuracy reads, Merqury estimates base-level accuracy and completeness. For trios, Merqury can also evaluate haplotype-specific accuracy, completeness, phase block continui ...[more]
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