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Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study.


ABSTRACT: Viruses are important agents in lung function deterioration in Cystic Fibrosis (CF). To date, no standard operating procedures (SOPs) have been established to determine which sampling method is the most effective for an optimal virological diagnosis of respiratory viral infections in CF. Here we investigated the performances of two sampling sites, sputum samples versus nasopharyngeal (NP) swabs, for thirty participants from three CF centres presenting an acute respiratory infection. Sputum and NP samples were simultaneously collected and multiplex PCR targeting 16 to 18 viruses were performed. Viruses were detected for 18/30 patients (60%). A high concordance between the sputum and NP samples was observed in 25 (83%) paired samples of which 13 tested positive and 12 tested negative. These results highlighted the relevance of sputum sampling for diagnostic of respiratory viruses in CF, which is less invasive and better accepted by CF patients than NP, and allows accurate bacterial detection.

SUBMITTER: Cardot-Martin E 

PROVIDER: S-EPMC7489228 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study.

Cardot-Martin Emilie E   Guillou-Guillemette Hélène Le HL   Berre Rozenn Le RL   Ramel Sophie S   Bihan Jean Le JL   Grenet Dominique D   Farfour Eric E   Troussier Françoise F   Urban Thierry T   Billard Lisa L   Pilorgé Léa L   Minoui-Tran Adissa A   Payan Christopher C   Munck Marie-Reine MR   Héry-Arnaud Geneviève G   Vallet Sophie S  

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 20200914 3


Viruses are important agents in lung function deterioration in Cystic Fibrosis (CF). To date, no standard operating procedures (SOPs) have been established to determine which sampling method is the most effective for an optimal virological diagnosis of respiratory viral infections in CF. Here we investigated the performances of two sampling sites, sputum samples versus nasopharyngeal (NP) swabs, for thirty participants from three CF centres presenting an acute respiratory infection. Sputum and N  ...[more]

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