Dataset Information

MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3.

ABSTRACT: Purpose:This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3). Methods:qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice. Results:MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells. Conclusion:Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3.

SUBMITTER: Sun S

PROVIDER: S-EPMC7490058 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3.

Sun Shengliang S Fu Lei L Wang Gen G Wang Jianli J Xu Liping L

Cancer management and research 20200908

<h4>Purpose</h4>This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving <i>pannexin 3</i> (<i>PANX3</i>).<h4>Methods</h4>qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. <i>PANX3</i> and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed t ...[more]

PMID: 32982413

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Dataset Information

MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3.

Publications

MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3.

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